Composite

Part:BBa_K1157012

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-14)

Pc-RhlR-pRhl-RhlR-luciferase

This is a biosensor that is built to sense C-4 AHL molecules, and uses luciferase as a reporter. It has a positive-feedback design to amplify signals. Our advisor has suggested that luciferase acts as a better quantitative measure reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1735
    Illegal BsaI.rc site found at 2325


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Categories
Parameters
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